04:05:2010 21:07 jm image autogenerated-by: DAG-Edit version 1.316 saved-by: ma11 date: Mon Feb 17 09:56:49 GMT 2003 version: $Revision: 1.4 $ Biological Imaging Methods CV This CV covers sample preparation, visualization and imaging methods. editor:Chris Woodcock (The Cell: An Image Library) corrections and additions to chris@bio.umass.edu image This is an OWL translation of an ontology whose native representational form is .obo. The translation was performed using the oboInOwl xslt library. For details, see http://www.berkeleybop.org/obo-conv.cgi dummy term image sample preparation method image chemically fixed tissue image coagulating-fixative fixed tissue image acetone fixed tissue image acrolein fixed tissue image ethanol fixed tissue image methanol fixed tissue image crosslinking-fixative fixed tissue image ethylene glycol-bis-succinimidyl succinate fixed tissue EGS image formaldehyde fixed tissue image glutaraldehyde fixed tissue image osmium tetroxide fixed tissue image cryofixed tissue image unfixed tissue image embedded tissue image tissue in plastic embedment image tissue in acrylic resin embedment image tissue in epoxy resin embedment image tissue in wax embedment image tissue in paraffin embedment image tissue in polyester wax embedment image tissue in polyethylene glycol embedment image unembedded tissue image whole mounted tissue image living tissue Unfixed fully hydrated tissue, typically employed to monitor dynamic changes in the living state. XX:ISBN978-087969683-2 http://purl.org/obo/owl/XX#XX_ISBN978-087969683-2 live-cell imaging image sectioned tissue image cryostat-sectioned tissue image hand-sectioned tissue image microtome-sectioned tissue image vibratome-sectioned tissue image visualization method image endogenous substrate, non-vital dye image diagram image HA peptide tag image cresyl fast violet image endogenous substrate, diaminobenzidine image eosin image ethidium bromide image fuchsin image giemsa image hematoxylin image methyl green image methyl violet image nitro blue tetrazolium chloride image nuclear fast red image orcein image propidium iodide PI image SYTOX Green image toluidine blue image endogenous substrate, vital dye image acridine orange AO image Hoechst 33342 HO342 Hoechst 33342 image cationic colloidal gold image CMX rosamine (Mitotracker Red) Mitotracker X image DiOC6 (3,3'-dihexyloxacarbocyanine iodide) DiOC image 4',6-diamidino-2-phenylindole (DAPI) DAPI image lucifer yellow image methylene blue image Nile blue A image Nile red image pyronine image rhodamine 123 image rhodamine B image SYTO Blue image SYTO Green image SYTO Orange image SYTO Red image YO-PRO-1 image endogenous substrate, dye conjugate image endogenous substrate, acidotropic-fluor conjugate LysoTracker image endogenous substrate, lectin-fluor conjugate image endogenous substrate, lectin-gold conjugate image taxol image endogenous substrate, phalloidin-fluor conjugate image exogenous label image genetically encoded enzyme image beta-galactosidase lacZ, X-gal image beta-glucuronidase GUS, X-gluc image genetically encoded tag image EBFP BFP image ECFP CFP image EGFP GFP image Aequorea victoria red fluorescent protein tag RFP image EYFP YFP image DsRed DsRed image c-MYC peptide tag image FLAG peptide tag image intracellular filling visualization of the connected regions of a cell by filling with a label XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image tissue in vitreous ice embedment Fully hydrated tissue rapidly frozen to produce vitrified water, and maintained continuously below the glass transition temperature of water (approximately -137 deg C PubMed:PMID:_3043536_"Cryo-electron_microscopy_of_vitrified_specimens._Dubochet_J http://purl.org/obo/owl/PubMed#PubMed_PMID:_3043536_"Cryo-electron_microscopy_of_vitrified_specimens._Dubochet_J Adrian_M:NULL http://purl.org/obo/owl/Adrian_M#Adrian_M_NULL Chang_JJ:NULL http://purl.org/obo/owl/Chang_JJ#Chang_JJ_NULL Homo_JC:NULL http://purl.org/obo/owl/Homo_JC#Homo_JC_NULL Lepault_J:NULL http://purl.org/obo/owl/Lepault_J#Lepault_J_NULL McDowall_AW:NULL http://purl.org/obo/owl/McDowall_AW#McDowall_AW_NULL Schultz_P._Q_Rev_Biophys._1988_May;21(2):129-228" http://purl.org/obo/owl/Schultz_P._Q_Rev_Biophys._1988_May;21(2)#Schultz_P._Q_Rev_Biophys._1988_May;21(2)_129-228" tissue in amorphous ice embedment image fluor labeled RNA fill image 5-SFX fluorescein RNA fill image X-rhodamine RNA fill image permeabilized tissue Tissue treated to make cell membranes permeable, typically for the purpose of allowing access by exogenous stains or substrates. XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image fluor labeled protein fill image fluor conjugated actin fill image Alexa Fluor 488 actin fill image Alexa Fluor 568 actin fill image fluoresceine actin fill image rhodamine actin fill image phalloidin image Alexa Fluor 350 phalloidin fill image Alexa Fluor 488 phalloidin fill image eosin phalloidin fill image fluoresceine phalloidin fill image Oregon Green 488 phalloidin fill image Oregon Green 514 phalloidin fill image rhodamine phalloidin fill image Texas Red-X phalloidin fill image fluor conjugated tubulin fill image Oregon Green 514 tubulin fill image X-rhodamine tubulin fill image probe for lipid image DiA image DiD image DiI image DiO image DiR image miscellaneous fill image biotin ethylenediamine fill Neurobiotin image cobalt fill image silver-intensified cobalt fill image metabolically incorporated radioisotope image [14]C image [32]P image [33]P image [35]S image [3]H image label conjugated to probe image glycerol permeabilized tissue made permeable by the action of glycerol XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref glycerinated tissue image labeled primary antibody image primary antibody-enzyme conjugate, substrate image alkaline phosphatase image primary antibody-alkaline phosphastase conjugate, DAB image primary antibody-alkaline phosphastase conjugate, ELF 97 phosphate image primary antibody-alkaline phosphastase conjugate, Vector Black image primary antibody-alkaline phosphastase conjugate, Vector Blue image primary antibody-alkaline phosphastase conjugate, Vector Red image horseradish peroxidase image primary antibody-horseradish peroxidase, 4-chloro-1-naphthol image primary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole image primary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine image primary antibody-horseradish peroxidase, Vector NovaRed image primary antibody-horseradish peroxidase, Vector SG image primary antibody-horseradish peroxidase, Vector VIP image primary antibody-fluor conjugate image primary antibody-aminomethylcoumarin acetate AMCA image primary antibody-Cascade blue image primary antibody-cyanine Cy2 image primary antibody-fluorescein isothiocyanate FITC image primary antibody-indocarbocyanine Cy3 image primary antibody-indodicarbocyanine Cy5 image primary antibody-rhodamine red-X RRX image primary antibody-tetramethyl rhodamine isothiocyanate TRITC image primary antibody-Texas Red TR image primary antibody-gold conjugate image primary antibody plus labeled secondary antibody image secondary antibody-enzyme conjugate, substrate image acid phosphatase image secondary antibody-alkaline phosphastase conjugate, DAB image secondary antibody-alkaline phosphastase conjugate, ELF 97 phosphate image secondary antibody-alkaline phosphastase conjugate, Fast Blue B image secondary antibody-alkaline phosphastase conjugate, Fast Red image secondary antibody-alkaline phosphastase conjugate, INT image secondary antibody-alkaline phosphastase conjugate, Magenta Phos image secondary antibody-alkaline phosphastase conjugate, NABP image secondary antibody-alkaline phosphastase conjugate, NAGP image secondary antibody-alkaline phosphastase conjugate, NAMP image secondary antibody-alkaline phosphastase conjugate, NATP image secondary antibody-alkaline phosphastase conjugate, NBT image secondary antibody-alkaline phosphastase conjugate, Vector Black image secondary antibody-alkaline phosphastase conjugate, Vector Blue image secondary antibody-alkaline phosphastase conjugate, Vector Red image secondary antibody-alkaline phosphastase conjugate, BCIP image secondary antibody-beta-galactosidase, X-Gal image secondary antibody-beta-glucuronidase, X-gluc image esterase image secondary antibody-esterase, Naphthol-AS-D-chloroacetate image glucose oxidase image secondary antibody-glucose oxidase, TNBT image secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine image secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine silver enhanced image secondary antibody-horseradish peroxidase, 4-chloro-1-naphthol image secondary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole image secondary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine image secondary antibody-horseradish peroxidase, Vector NovaRed image secondary antibody-horseradish peroxidase, Vector SG image secondary antibody-horseradish peroxidase, Vector VIP image secondary antibody-phosphatase, Naphthol-AS-BI-phosphate image secondary antibody-fluor conjugate image secondary antibody-Alexa Fluor conjugate image aminomethylcoumarin AMCA image Cascade blue image secondary antibody-cyanine conjugate Cy2 image secondary antibody-diaminotriazinylaminoflorescein conjugate DTAF image secondary antibody-fluorescein isothiocyanate conjugate FITC image secondary antibody-indocarbocyanine conjugate Cy3 image secondary antibody-indodicarbocyanine conjugate Cy5 image secondary antibody-lissamine rhodamine sulfonyl chloride LSRC image secondary antibody-rhodamine red-X conjugate RRX image secondary antibody-tetramethyl rhodamine isothiocyanate conjugate TRITC image secondary antibody-Texas red conjugate TR image gold image labeled nucleic acid probe image DNA probe image biotin labeled DNA probe image digoxigenin labeled DNA probe image fluor labeled DNA probe image BODIPY TR-14-dUTP labeled DNA probe image bromodeoxyuridine labeled DNA probe image fluoresceine-12-dUTP labeled DNA probe image Oregon Green 488-5-dUTP labeled DNA probe image radioisotope labeled DNA probe image [32]P labeled DNA probe image [33]P labeled DNA probe image RNA probe image biotin labeled RNA probe image digoxigenin labeled RNA probe image fluoroscein labeled RNA probe image radioisotope labeled RNA probe image [32]P labeled RNA probe image [33]P labeled RNA probe image imaging method image graphic illustration image computer graphic image black and white graphic image color graphic image grey scale graphic image camera lucida assisted graphic image free hand graphic image line art graphic image charcoal pencil graphic image colored pencil graphic image graphite pencil graphic image pen and ink graphic black and white graphic image painted graphic image acrylic painted graphic image oil painted graphic image pastel painted graphic image watercolor painted graphic image macroscopy methods for imaging objects large enough to be observed by the unaided eye XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image microscopy methods for forming images of objects too small to be observed with the unaided eye XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image wide field light micrograph image bright-field microscopy image dark-field microscopy imaging with rejection of the unscattered illumination (removal of the zero order component of the diffracted wave) XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image differential interference contrast microscopy DIC Nomarski image fluorescence microscopy image phase contrast microscopy image polarization microscopy image time lapse microscopy image narrow field light micrograph image confocal microscopy image single-spot confocal microscopy image spinning disk confocal microscopy image two-photon laser scanning micrograph image multi-photon microscopy image modes of electron microscopy image scanning electron microscopy (SEM) image is formed by scanning the surface of the specimen with a beam of electrons in a raster pattern XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image transmission electron microscopy (TEM) image atomic force microscopy the scanning probe is maintained at a fixed distance above the surface e of the specimen by van der Waals forces XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image X-ray micrograph image montage image detergent permeabilized Membrane permeabilization by the action of ionic or non-ionic detergents XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image solvent permeabilized permeabilization due to extraction of lipid from membranes by an organic solvent such as acetone or methanol. XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image saponin permeabilized Saponin, a detergent-like molecule, preferentially forms holes in membranes that contain cholesterol. XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> saponized tissue image recorded image methods and devices used for capturing an image of a real object; distinct from images created by artwork XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> micrograph photograph image portrayed image methods and devices used for creating images by some form of artwork, as distinct from capturing images by means of some recording device XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref artwork drawing image animation A form of graphical illustration that changes with time to give a sense of motion or represent dynamic changes in the portrayal. XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image illumination methods sources and methods for illumination XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image detection methods media and devices employed for recording the alterations in the illumination that result from its interaction with the sample XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image imaged parameters properties of the sample that vary with location or time and thereby provide contrast in the image XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image contrast-enhancing methods image illumination by photons image illumination by electrons image illumination by acoustic waves image illumination by neutrons image illumination by ions image widefield illumination illumination that covers the entire field of view of the image-forming lens XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image narrowfield illumination illumination restricted to a small portion of the field of view of the imaging lens; often one or more diffraction limited spots XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image coherent illumination image incoherent illumination image nearfield illumination image farfield illumination image continuous illumination image pulsed illumination illumination that varies with time; often achieved with pulsed lasers for the purpose of generating very-high peak illumination power XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image oblique illumination image single point scanning illumination by a single spot of light that is scanned across the field of view XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image multiple point scanning illumination by multiple points of light simultaneously XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image spinning disk scanning scanning by multiple points of light arising from pinholes in a spinning disk that is illuminated by a widefield source XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image array scanning Multiple points of illumination generated by a fixed array of sources. XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image hollow-cone illumination illumination parallel or at small angles to the optical axis is attenuated or blocked by a stop XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image anaxial illumination Illumination is distributed asymmetrically around the optic axis, typically achieved by a non-circular stop placed below the lower lens element and aperture of the condenser. NULL:NULL http://purl.org/obo/owl/NULL#NULL_NULL image darkfield illumination illumination restricted to angles that exceed the acceptance angle of the image-forming lens XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image Rheinberg illumination illumination at angles less than the acceptance angle of the image-forming lens is attenuated by a filter to give a colored background of unscattered light; higher angle illumination may be filtered to give a contrasting color XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image charge coupled device (CCD) image photomultiplier tube (PMT) image photodiode image avalanche photodiode (APD) image silicon intensified target tube (SIT) image intensified SIT (ISIT) image intensified CCD (ICCD) image electron multiplying CCD (EMCCD) image electron bombardment CCD (EBCCD) image film image complementary metal oxide semiconductor (CMOS) image piezo-electric device image wide-field detection image narrow-field detection image absorption image inelastic scattering image elastic scattering image refractive index image refractive index gradient image birefringence image stiffness image electron density image fluorescence emission image fluorescence lifetime image fluorescence polarization image chemical composition image elevation image resolution-enhancing methods methods for increasing the resolution beyond the classical Abbe diffraction limit XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image optical methods image computational methods image in-focus phase contrast image defocus phase contrast image Wollaston prism a birefringent optical element typically used to split polarized illumination into two sets of parallel but displaced rays XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image Hoffman modulation image single sideband edge enhancement (SSBE) image interference reflection contrast (IRM) image interference contrast image polarization contrast image structured illumination microscopy (SIM) image point-localization methods image stimulated emission depletion (STED) image PALM (photoactivation localization microscopy) image STORM (stochastic optical reconstruction microscopy) image FIONA (fluorescence imaging with one nanometer accuracy) image DOPI (defocussed orientation and position imaging) image PAINT (point accumulation for imaging in nanoscale topography) image saturated structured-illumination microscopic (SSIM) structured illumination used at sufficiently high power to cause significant ground-state depletion of the imaged fluorophore, thus allowing for non-linear resolution enhancement XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image UV/visible/IR illumination image X-ray illumination image microscopy with lenses image scanning probe microscopy imaging by means of a physical probe that passes over the sample XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image modes of light microscopy image scanning tunneling microscopy image nearfield scanning optical microscopy (ANSOM) image ANSOM Aperture-less NSOM. XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image polarized light illumination image orientation-independent polarization microscopy image polarization-sensitive detection image modified objective lens uses an objective with extra components to manipulate the wave front in the plane of the objective aperture XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image linear methods a computational method that is linear in the mathematical sense, meaning that image intensity is conserved and the data remain suitable for all quantitative analyses XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image non-linear methods computational method that are mathematically non-linear; image intensity is not conserved, and the data are in general not suitable for quantitative analyses of pixel values XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image Wiener filter image 3D-deconvolution image no-neighbors deblurring image nearest-neighbors deblurring image constrained iterative deconvolution image maximum likelihood deconvolution image blind deconvolution image exhaustive photon reassignment image light-sheet illumination illumination in the form of a thin sheet of light directed perpendicular to the optic axis XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image FLIP Fluorescence loss in photobleaching; a method of determining whether two populations of fluorophore are in diffusive communication with one another by observing the decrease in brightness of one population when the other population is subjected to continuous photobleaching XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image FRAP Fluorescence loss in photobleaching; a method of determining the rate and extent of exchange between a localized population of fluorophore and a non-localized diffusible pool, by observing the rate of recovery of brightness of the localized population after photobleaching XX:-new-dbxref- http://purl.org/obo/owl/XX#XX_-new-dbxref- image FRET Fluorescence (or Forster) resonance energy transfer; a method of estimating the distance between two fluorophores by measuring the extent of non-radiative energy transfer between the two. Experimentally, this involves selective excitation of one while observing emission from the other. XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image FLIM image SPIM single (or selective) plane illumination XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image CARS coherent Raman anti-Stokes microscopy XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image OCT optical coherence tomography; an interferometric method of imaging using back-scattered photons (elastic scattering) XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image secondary_electron imaging image created using the electrons generated by ionization of a sample due to inelastic scattering of the primary beam of radiation XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image back-scattered_electron imaging imaged formed using electrons undergoing elastic scattering at very high angles, with emission of characteristic X-rays XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image detection of photons image detection of electrons image detection of acoustic waves image detection of current induced in the specimen image UV/visible/IR photons image X-ray photons image scanning-transmission electron microscopy image EELS imaging image EDAX imaging image specimen_modifying_illumination Illumination used for the purpose of inducing some observable change in the specimen during the course of an imaging session. This illumination may or may not be the same as the illumination used for imaging. Typically used in conjunction with live-cell imaging XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image photo-bleaching illumination image photo-ablation illumination image photo-switching illumination image photo-activation illumination image uncaging illumination image trapping illumination image emission-depletion illumination image slit-scanning illumination illumination in the shape of a thin line that is swept over the field of view XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image slit-scan confocal illumination and detection via a thin slit XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image array-scan confocal Fixed array of sources focused to an array of spots that is swept over the specimen in raster fashion XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image spot detectors image area detectors image visualization of contiguous regions methods of visualization that differentiate between different regions or surfaces of the specimen based on contiguity XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image visualization by chemical attribute methods of visualization that differentiate regions of the specimen on the basis of their chemical properties XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image shadowing and plating visualization by depositing a label on the surface of a specimen uniformly (plating), or from a particular direction so that surface topography becomes visible (shadowing) XX:<new_dbxref> http://purl.org/obo/owl/XX#XX_<new_dbxref> image negative staining visualization by exclusion of a stain in which the specimen is immersed; compare with "positive staining. XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image sequence-specific nucleic acid probes image antibodies image lectins image small-molecule probe image fluorescent dextran fill image fluorescent protein tag image probes for nucleic acid image small genetically encoded tags image probe for protein image organelle-specific probe image macromolecular probe image probe for mitochondria image probe for endoplasmic reticulum image probe for Golgi image probe for lysosomes image stain with broad specificity positive staining image tetracysteine tag image Hoechst 33258 image genetically encoded biotin tag image freeze_fracture/freeze_etch image fluorescent proteins derived from Aequorea victoria image membrane-permeant probe image membrane impermeant probe image acridine homodimer image 7-amino-actinomycin D image conconavalin A image wheat germ agglutinin image Phaseolus vulgaris lectin PHA-L) image Arachis hypogaea (PNA) image Helix pomatia agglutinin image soybean agglutinin image cholera toxin B image peptide-nucleic acid probe image fluorescent proteins derived from Anthozoa image optical highlighters image Blue fluorescent proteins from Aequorea victoria image Cyan fluorescent proteins from Aequorea image Green fluorescent proteins from Aequorea image DiIC16 image Yellow fluorescent proteins from Aequorea image Alexa Fluor 488 image Alexa Fluor 546 image Alexa Fluor 568 image Alexa Fluor 555 image Alexa Fluor 594 image Alexa Fluor 610 image Alexa Fluor 633 image Alexa Fluor 647 image Cy2 image Cy3 image Cy5 image Fluorescein (FITC) image Rhodamine image TexasRed fluorophore with ex/em 589/615 XX:NULL http://purl.org/obo/owl/XX#XX_NULL image Tetramethyl rhodamine (TRITC) fluorophore derivative of rhodamine with ex/em 547/572 XX:NULL http://purl.org/obo/owl/XX#XX_NULL image fluorescent labels image enzyme labels image electron dense labels image biotin image affinity for specific proteins image non-specific protein affinity image AzuriteFP image SiriusFP image non-immunological protein probes image alpha-bungarotoxin image botulinum toxin image tetrodotoxin image batrachatoxin image CeruleanFP image CyPet image SCFP image EmeraldFP image SuperfolderFP image T-SapphireFP image CitrineFP image SYFP image TopazFP image VenusFP image yPet image Blue fluorescent proteins from Anthozoa image Cyan fluorescent proteins from Anthozoa image Green fluorescent proteins from Anthozoa image Orange fluorescent proteins from Anthozoa image Red fluorescent proteins from Anthozoa image Far-red fluorescent proteins from Anthozoa image Yellow fluorescent proteins from Anthozoa image mTagBFP image AmCyanFP image mCyFP image mTFP1 image TagCFP image AQ143 image HcRed-tandemFP image KatushkaFP image mKateFP image mKate2FP image mPlumFP image tdKatuskaFP image tdRFP639 image AceFP image Azami GreenFP image CopGFP image mWasabiFP image TagGFP image TagGFP2 image ZsGreenFP image DsRed-ExpressFP image DsRed-Express2FP image DsRed-MaxFP image DsRed-monomerFP image DsRed2FP image dTomatoFP image Kusabira OrangeFP image Kusabira OrangeFP2 image mOrangeFP image mOrange2FP image TagRFP image TagRFP-T image tdTomatoFP image turboRFP image AsRed2FP image eqFP611 image HcRed1FP image JRedFP image mAppleFP image mCherryFP image mRasberryFP image mRFP1 image mRubyFP image mStrawberryFP image tdRFP611 image PhiYFP image TagYFP image TurboYFP image ZsYellowFP image fluorescent protein timers image photoactivatable fluorescent proteins image photoconvertible fluorescent proteins image photoconvertible/photoswitchable fluorescent proteins image photoswitchable fluorescent proteins image DsRed-ES FP image Fast-FT image Medium-FT image Slow-FT image PA-GFP image PA-CFP image PA-mCherry1FP image PA-mRFP image Phamret image Dendra2FP image dEOSFP image KaedeFP image mEOS2FP image mKikGR image wtEOSFP image wtKIikGR image IrisFP image bsDronpa image Dronpa image Dronpa-3 image E2GFP image KFP1 image mTFP0.7 image PadronFP image rsCherryFP image rsCherryRevFP image rsFastLimeFP image optically-dense stain image electron-dense stain image uranyl salt image lead salt image osmium tetroxide image potassium permanganate image uranium compound image molybdenum compound image tungsten compound image aurothioglucose image other negative stain image other radioisotope image isolated subcellular component A fragment of a cell, subcellular organelle, or macromolecular complex. XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image secondary electron generation electrons generated by inelastic scattering of other radiation, the primary radiation. XX:NULL http://purl.org/obo/owl/XX#XX_NULL image critical_point dried specimen specimen from which water has been removed by a process that avoids liquid-gas and solid-gas phase transitions. XX:NULL http://purl.org/obo/owl/XX#XX_NULL image unprocessed raw data data as acquired, with no alterations that change the pixel values. XX:NULL http://purl.org/obo/owl/XX#XX_NULL image lyophilized specimen specimen from which water has been removed by evaporation under vacuum at a temperature below freezing. XX:NULL http://purl.org/obo/owl/XX#XX_NULL image X-Rhodamine fluorophore derivative of rhodamine with ex/em 580/605 XX:NULL http://purl.org/obo/owl/XX#XX_NULL image Biological Imaging Methods image makes_use_of the child is an essential component of the parent device, or plays an essential role in performance of the parent function XX:newdbxref http://purl.org/obo/owl/XX#XX_newdbxref image Is a image